Authors

Modern cryopreservation methods for umbilical cord blood stem cells in the public registry of donors

UDC 62-9

Modern cryopreservation methods for umbilical cord blood stem cells in the public registry of donors

Ivolgin D. A. , Smolyaninov A. B. , Bagautdinov Sh. M. , Korovina K. V.

Abstract
The changes of qualitative characteristics are analysed for 85 umbilical cord blood samples, as their nucleus-containing cells have been tested in the process of cryoconservation, defreezing and removal of the cryoprotectant. The method of cryopreservation used for umbilical cord blood, and the technique of purging cryoprotectant from the cord blood after thawing by means of an automated system are found to provide for the best quality in an umbilical cord blood samples.

Keywords: cryopreservation, cryoprotectant, haemapoietic stem cells, umbilical cord blood, viability, long-term storage

All references

1. Gluckman E., Broxmeyer H. E., Auerbach A. D. et al. Hematopoietic reconstitution in a patient with Fanconi anemia by means of umbilical-cord blood from an HLA-identical sibling // N Engl J Med. 1989. № 321 (17).

2. Rubinstein P. et al. Processing and cryopreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution // Proc. Nati. Acad. Sci. USA. 1995. № 92 (22).

3. Chow R. et al. Cell recovery comparison between plasma-depletion/reduction- and red cell reduction processing of umbilical cord blood // Cytotherapy. 2011. № 13 (9).

4. Hunt C. J., Armitage S. E., Pegg D. E. Cryopreservation of umbilical cord blood: 2. Tolerance of CD34(+) cells to multimolar dimethyl sulphoxide and the effect of cooling rate on recovery after freezing and thawing // Cryobiology. 2003. № 46 (1).

5. Zenhausern R., Tobler A., Leoncini L. et al. Fatal cardiac arrhythmia after infusion of dimethyl sulfoxide-cryopreserved hematopoieticstem cells in a patient with severe primary cardiac amyloidosis and end-stage renal failure // Ann Hematol. 2000. № 79 (9).

6. Hubel A. et al. Cryopreservation of cord blood after liquid storage // Cytotherapy. 2003. № 5 (5).

7. Shlebak A. A. et al. Optimal timing for processing and cryopreservation of umbilical cord haematopoietic stem cells for clinical transplantation // Bone Marrow Transplantation. 1999. № 23 (2).

8. Broxmeyer H. E. High-efficiency recovery of functional hematopoietic progenitor and stem cells from human cord blood cryopreserved for 15 years // Proc. Nati. Acad. Sci. USA. 2003. № 100 (2).

9. Broxmeyer H. E. Experimental basis of cord blood transplantation // Bone Marrow Transplantation. 2009. № 44 (10).

Read the full article