Authors

Organizational aspects of red blood cells cryopreservation in the activities of blood service in Russian Federation

UDC 615.38.012:612.111: 615.014.41

Organizational aspects of red blood cells cryopreservation in the activities of blood service in Russian Federation

Chechetkin A. V., Danilchenko V. V., Volkova S. D., Makeev A. B., Soldatenkov V. E., Kiryanova G. Yu., Kasyanov A. D., Golovanova I. S.

Abstract
In article the factors of cryopreserved erythrocytes preparation and their use for clinical purposes in Russian medical institutions were analyzed. The tendencies of red blood cells cryopreservation development at stations and departments of blood transfusion (blood centers) in the Federal Districts of the Russian Federation were revealed. The main developments on cryopreservation of red blood cells are presented here: methods of freezing the red blood cells at ultra-low and moderately low temperatures protected by reduced concentration of glycerol (15-20%), with the optimized procedure of washing up the erythrocytes (double centrifugation and using solutions of sodium chloride in decreasing concentration); method of deferred washing up of erythrocytes after defrosting and storing at 4 deg C; the technology of procurement and stockpiling the leukoreduced quarantinized erythrocytes for immunological and infectious safety of transfusions.

Keywords: blood service, erythrocytes cryopreservation, delayed erythrocytes washing

All references

Chechetkin A.V., Vilyaninov V.N., Kaleko S.P. et al. Organizational aspects of the application of low-temperature technology in modern production transfusiology. Vestnik Mezhdunarodnoi akademii kholoda. 2005. No.2. P. 34-39. (in Russian)
Selivanov E.A., Chechetkin A.V., Grigorian M.W., Makeev A.B. State and problems of cryopreservation of red blood cells in the blood service of the Russian Federation. Transfusiology. 2012. No.2. P.14-20. (in Russian)

3. Uyba V.V. Program development is a historical chance for the blood service. Transfusiology. 2009. Vol.13, No 2. P. 4-13. (in Russian)

4. Grachev A.E., Nakostoev I.M., Gribanova E.O. et el. Clinical efficacy of transfusions of cryopreserved erythrocytes of different storage durations. Gematologiya i Transfuziologiya. 2014. Vol.59, suppl.1. P. 87. (in Russian)

5. Vilyaninov V.N., Kaleko S.P., Bagautdinov Sh.M. et al. Reserve components of the blood and hematopoietic cells using low temperatures: the state and prospects of implementation in the interests of victims in emergency conditions. Vestnik Mezhdunarodnoi akademii kholoda. 2014. No 4. P. 48-53. (in Russian)

6. Bagautdinov Sh.M., Vilyaninov V.N. et al. Influence of deep freezing on cellular structure of the human red blood cells. Vestnik Mezhdunarodnoi akademii kholoda. 2014. No 2. p. 41-44. (in Russian)

7. Chechetkin A.V., Hubulava G.G., Matveev S.A., Viljaninov V.N. Autohemotransfusions in surgical practice. Efferent Therapy. 2004. Vol.10., No 3. P. 87-92. (in Russian)

8. Gudkov A.G., Onufrievich A.D., Kayumova L.I. et al. Defroster cryopreserved blood products "Plasmoterm-4”: ensure the accuracy of the process heat treatment. Biomedical technologies and radioelectronics. 2007. No 6. P. 39-43. (in Russian)

9. Vilyaninov V.N., Vashchenko V.I., Kurcova M.V. et al. Cryopreservation of human erythrocytes at low temperature -80 °C in polymeric containers of OAO "Synthesis". Vestnik of the blood service of Russia. 2011. No 1. P.17-22. (in Russian)

10. Bagautdinov Sh.M., Copelets A.V., Chechetkin A.V. et al. Use of new polymer cryo containers for freezing red blood cells at ultra-low temperature. Gematologiya i Transfuziologiya. 2009. Vol. 54, No 1. P.25. (in Russian)

11. Sareneva I.M., Haimila K.E., Korhonen A.E. et al. Use of frozen red blood cell units in Finland. Vox Sang. 2013. Vol. 105, Suppl. 1. P. 123-124.

12. Hansen A., Yi Q.L., Acker J.P. Quality of red blood cells washed using the ACP 215 cell processor: assessment of optimal pre - and postwash storage times and conditions. Transfusion. 2013. Vol. 53, No 8. P. 1772-1779.

13. Melnikova V.N., Zhamaletdinova T.V., Kiryanova G.Yu. Prolonged storage at +4 ^oC of cryopreserved and defrozen erythrocytes. Gematologiya i Transfuziologiya. 1995. Vol. 40, No 3. P. 29-32. (in Russian).

14. Melnikova V.N., Zhamaletdinova T.V., Kiryanova G.Yu. A modified method of deglycerinization of defrosted red blood cells, cryopreserved in liquid nitrogen. Gematologiya i Transfuziologiya. 1996. Vol. 41, No 1. P. 39-41. (in Russian)

15. Melnikova V.N., Selivanov E.A., Kiryanova G.Yu. et al. The value of leukofiltration in cryopreservation of red blood cells for the purpose of their quarantine. Vestnik of the blood service of Russia. 2010. No 4. P. 3-7. (in Russian).

16. Li T.-D., Gао J., Szoszkiewicz R. et al. Structured and viscous water in subnanometer gaps. Phys. Rev. 2007. Vol. 75. P. 115-415.

17. Ebner А., Schillers Н., Hinterdorfer Р. Normal and pathological erythrocytes studied bу atomic force microscopy. Methods Моl. Biol. 2011. Vol. 736. P. 223-241.