Cryopreservation of erythrocytes at –40 оС and –80 оС
DOI: 10.21047/1606-4313-2017-16-1-72-78
UDC 611–018.51 : 615.361.018.46–014.41
Kiryanova G. Yu., Volkova S. D., Kasyanov A. D., Grishina G.V., Golovanova I. S., Chechetkin A. V.
Keywords: cryopreservation, washed defrozen erythrocytes, deglycerinization, moderate low temperatures, morphophysiology properties.
UDC 611–018.51 : 615.361.018.46–014.41
Cryopreservation of erythrocytes at –40 оС and –80 оС
For citation: Кирьянова Г.Ю., Волкова С.Д., Касьянов А.Д., Гришина Г.В., Голованова И.С., Чечеткин А.В. Криоконсервиро-вание эритроцитов при температурах –40 оС и –80 оС // Вестник Международной академии холода. 2017. № 1. С. 72-78
Abstract
Results of cryopreservation of erythrocytes at the temperatures of –40 °C and –80 °C are presented in the article. Standard dose of erythrocytes decryopreservated after storage within 2-5 months at –40 °C was shown to be much safer and more functionally full unlike its behavior at –80 °C. The difference between low-temperature storage at –40°C and –80°C was statistically significant in terms of hemolysis level, hemoglobin content in the dose and the quantity of osmotically unstable erythrocytes. The hemolysis rate in suspensions of washed defrozen erythrocytes cryopreserved at –40 °C and at –80 °C was 0.06±0.011 and 0.53±0.125 respectively. The washed defrozen erythrocytes stored at –80 °C were shown to exhibit low hemoglobin content (18.4±1.69 g) and percentage of preserved cells (31.1±3.09) in the dose. A meaningful dependence of these indicators on a method of defreezing (in water bath at 40 °C or in the SAHARA device), and also on a method of freezing to –80 °C (linear or two-level) was not found. At the same time the content of hemoglobin in a dose of the deglycerinizated erythrocytes stored at –40 °C was 49.5±1.88 g., safety of erythrocytes – 83.8±4.09% that meets modern criteria of this medium applicability.
Abstract
Results of cryopreservation of erythrocytes at the temperatures of –40 °C and –80 °C are presented in the article. Standard dose of erythrocytes decryopreservated after storage within 2-5 months at –40 °C was shown to be much safer and more functionally full unlike its behavior at –80 °C. The difference between low-temperature storage at –40°C and –80°C was statistically significant in terms of hemolysis level, hemoglobin content in the dose and the quantity of osmotically unstable erythrocytes. The hemolysis rate in suspensions of washed defrozen erythrocytes cryopreserved at –40 °C and at –80 °C was 0.06±0.011 and 0.53±0.125 respectively. The washed defrozen erythrocytes stored at –80 °C were shown to exhibit low hemoglobin content (18.4±1.69 g) and percentage of preserved cells (31.1±3.09) in the dose. A meaningful dependence of these indicators on a method of defreezing (in water bath at 40 °C or in the SAHARA device), and also on a method of freezing to –80 °C (linear or two-level) was not found. At the same time the content of hemoglobin in a dose of the deglycerinizated erythrocytes stored at –40 °C was 49.5±1.88 g., safety of erythrocytes – 83.8±4.09% that meets modern criteria of this medium applicability.
Keywords: cryopreservation, washed defrozen erythrocytes, deglycerinization, moderate low temperatures, morphophysiology properties.
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