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The effect of enzyme preparations on extracting the pigments from plant raw materials

DOI: 10.17586/1606-4313-2019-18-1-64-68
UDC 543.054

The effect of enzyme preparations on extracting the pigments from plant raw materials

Adadi P., Filippova D.S., Barakova N.V.

For citation: Adadi P., Filippova D.S., Barakova N.V. The effect of enzyme preparations on extracting the pigments from plant raw materials. Vestnik Mezhdunarodnoi akademii kholoda. 2019. No 1. p.

Abstract
The resulting solution was filtered on a Buchner funnel through filter paper and glass wool under a vacuum of 0.2 bar, followed by washing the residue on the filter with ethyl alcohol. The quantitative content of chlorophyll-a, chlorophyll-b, and carotenoids was determined spectrophotometrically using an AgilentCary 60 UV-Vis spectrophotometer (Agilent MY15510018, USA) with a polymer cuvette at the wavelength of 300-700 nm.As a result of the experiments it was found that Fructozym® MA played a decisive role in the yield of chlorophylls, and cellulase-A in combination with Fructozym® MA and Fructozym® BE - in the yield of carotenoids. An inverse correlation between the yield of carotenoids and chlorophylls was also established. The results obtained are recommended to be taken into account when developing technologies for the production of biologically active additives containing such important components as chlorophylls and carotenoids used as powerful antioxidant and anticarcinogenic agents, as well as natural dyes.

Keywords: pigments of plant raw materials, chlorophylls, carotenoids, enzyme preparations, extraction of organic pigments.

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