Study of an enzyme preparation from the digestive organs of freshwa-ter brook trout
UDC 577.151.63
Bazhenov Elisei A., Baydalinova L.S., Grimm T.
Keywords: proteolytic enzymes, brook trout, secondary raw materials, fish digestive organs, meat substrates, fish collagen-containing substrates, gel-penetrating chromatography, molecular weigh.
Study of an enzyme preparation from the digestive organs of freshwa-ter brook trout
For citation: Bazhenov E.A., Baydalinova L.S., Grimm T. Study of an enzyme preparation from the digestive organs of freshwater brook trout. Journal of International Academy of Refrigeration. 2022. No 3. p. 27-38. DOI: 10.17586/1606-4313-2022-21-3-27-38
Abstract
The results of the study of the proteolytic activity of enzymes isolated from the digestive organs (intestines with stomachs) are presented common trout of freshwater freshwater aquaculture, evaluation of the effectiveness of hydrolysis of meat raw materials and fish collagen-containing by these enzymes. The molecular weight of the enzymes of the preparation from the digestive organs of trout was determined. The results of the efficiency of hydrolysis by the separated enzymes of the protein substrate were evaluated by the molecular weight of the fragments obtained during hydrolysis. Molecular weights were determined by exclusive gel chromatography when used as a stationary phase in a Sephadex G-75 Superfine chromatographic column. The experiments were carried out at the ANiMOX research laboratory in Berlin with the support of the German Environmental Foundation. Enzyme preparations were isolated from the digestive tract (intestines with stomach) of trout during initial autoproteolysis for partial destruction of enzyme-containing cells into a buffer. Enzyme preparations were isolated from the digestive tract (intestines with stomach) of trout during initial autoproteolysis for partial destruction of enzyme-containing cells in a buffer solution with a pH of 8.0 for 3.5 hours at 37 OC. The extract (enzyme solution), freed from the fat fraction and dense residue, was used for subsequent studies. The study of the composition of the enzyme preparation by gel-penetrating chromatography showed the presence of a bulk fraction with a molecular weight of 26 kDa and a higher molecular fraction with a molecular weight of 36-38 kDa. The study of the effectiveness of the effect of enzymes on meat and collagen-containing fish substrate (trout and bream swim bladders) showed a high hydrolytic ability. The study confirmed the possibility of using secondary raw materials of freshwater aquaculture facility trout brook to obtain hydrolytic enzymes. The presence of proteolytic enzymes pepsin, trypsin was found in the preparations, chymotrypsin, collagenase, and lipase was also detected.
Abstract
The results of the study of the proteolytic activity of enzymes isolated from the digestive organs (intestines with stomachs) are presented common trout of freshwater freshwater aquaculture, evaluation of the effectiveness of hydrolysis of meat raw materials and fish collagen-containing by these enzymes. The molecular weight of the enzymes of the preparation from the digestive organs of trout was determined. The results of the efficiency of hydrolysis by the separated enzymes of the protein substrate were evaluated by the molecular weight of the fragments obtained during hydrolysis. Molecular weights were determined by exclusive gel chromatography when used as a stationary phase in a Sephadex G-75 Superfine chromatographic column. The experiments were carried out at the ANiMOX research laboratory in Berlin with the support of the German Environmental Foundation. Enzyme preparations were isolated from the digestive tract (intestines with stomach) of trout during initial autoproteolysis for partial destruction of enzyme-containing cells into a buffer. Enzyme preparations were isolated from the digestive tract (intestines with stomach) of trout during initial autoproteolysis for partial destruction of enzyme-containing cells in a buffer solution with a pH of 8.0 for 3.5 hours at 37 OC. The extract (enzyme solution), freed from the fat fraction and dense residue, was used for subsequent studies. The study of the composition of the enzyme preparation by gel-penetrating chromatography showed the presence of a bulk fraction with a molecular weight of 26 kDa and a higher molecular fraction with a molecular weight of 36-38 kDa. The study of the effectiveness of the effect of enzymes on meat and collagen-containing fish substrate (trout and bream swim bladders) showed a high hydrolytic ability. The study confirmed the possibility of using secondary raw materials of freshwater aquaculture facility trout brook to obtain hydrolytic enzymes. The presence of proteolytic enzymes pepsin, trypsin was found in the preparations, chymotrypsin, collagenase, and lipase was also detected.
Keywords: proteolytic enzymes, brook trout, secondary raw materials, fish digestive organs, meat substrates, fish collagen-containing substrates, gel-penetrating chromatography, molecular weigh.
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