Technology of proteolytic enzymes production from the digestive or-gans of fish of coastal fisheries in the North-Western region
DOI: 10.17586/1606-4313-2023-22-1-66-77
UDC 577.151.63
Bazhenov Elisei A., Baidalinova L.S., Grimm T.
Keywords: proteolytic enzymes, pike perch, bream, secondary raw materials, fish digestive organs, parameters of the enzyme isolation process, liquid high-efficiency chromatography, gel-penetrating chromatog-raphy, molecular weight.
UDC 577.151.63
Technology of proteolytic enzymes production from the digestive or-gans of fish of coastal fisheries in the North-Western region
For citation: Bazhenov E.A., Baydalinova L.S., Grimm T. Technology of proteolytic enzymes production from the digestive organs of fish of coastal fisheries in the North-Western region. Journal of International Academy of Refrigeration. 2023. No 1. p. 66-77. DOI: 10.17586/1606-4313-2023-22-1-66-77
Abstract
The article presents the general chemical composition and activity of proteolytic enzymes of fish digestive organs of the Northwestern region (bream and walleye) in different periods of the year at different pH. Based on the data obtained, enzymes were isolated from the digestive organs of bream at pH 9.5 and walleye at pH 2.5. A technological scheme for the production process of proteolytic enzyme preparations from secondary raw materials of fish of the North-Western region is proposed. The study of the proteolytic activity of the obtained preparations showed the effectiveness of the proposed technology under the selected regimes and during optimal periods of the year.Experiments to refine the technology in the ANiMOX GmbH biotech company (Germany) have shown the possibility of extracting enzymes at a temperature of 35 °C. The enzyme preparations were purified by ion exchange chromatography using ion exchangers (Q-XL and SP-XL). The molecular weight of enzymes in the preparations was determined by the method of exclusive (gel-penetrating) chromatography using Sephadex G-75 Superfine.
Abstract
The article presents the general chemical composition and activity of proteolytic enzymes of fish digestive organs of the Northwestern region (bream and walleye) in different periods of the year at different pH. Based on the data obtained, enzymes were isolated from the digestive organs of bream at pH 9.5 and walleye at pH 2.5. A technological scheme for the production process of proteolytic enzyme preparations from secondary raw materials of fish of the North-Western region is proposed. The study of the proteolytic activity of the obtained preparations showed the effectiveness of the proposed technology under the selected regimes and during optimal periods of the year.Experiments to refine the technology in the ANiMOX GmbH biotech company (Germany) have shown the possibility of extracting enzymes at a temperature of 35 °C. The enzyme preparations were purified by ion exchange chromatography using ion exchangers (Q-XL and SP-XL). The molecular weight of enzymes in the preparations was determined by the method of exclusive (gel-penetrating) chromatography using Sephadex G-75 Superfine.
Keywords: proteolytic enzymes, pike perch, bream, secondary raw materials, fish digestive organs, parameters of the enzyme isolation process, liquid high-efficiency chromatography, gel-penetrating chromatog-raphy, molecular weight.
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