Comparative analysis of β-glucans received from fruit bodies and mycelium of basidiomycetes with IR-spectroscopy
DOI: 10.17586/1606-4313-2024-23-4-46-51
UDC 57.083.132
Razin Aleksandr N., Власова Е. Н., Vorobeychikov Evgeny V. , Leonova Natalia V., Konusova Valentina G., Shamtsyan M.M. , Frioui M., Kolesnikov Boris A., Kiprushkina E.I., Volkov Mikhail Yu.
Keywords: basidiomycetes, β-glucan, Pleurotus ostreatus, Phallus impudicus, enzymatic method, IR spectroscopy.
UDC 57.083.132
Comparative analysis of β-glucans received from fruit bodies and mycelium of basidiomycetes with IR-spectroscopy
For citation: Razin A.N., Vlasova E.N., Vorobeychikov E.V., Leonova N.V., Konusova V.G., Shamtsyan M.M., Frioui M., Kolesnikov B.A., Kiprushkina E.I., Volkov M.Yu. Comparative analysis of β-glucans received from fruit bodies and mycelium of basidiomycetes with IR-spectroscopy. Journal of International Academy of Refrigeration. 2024. No 4. p. 46-51. DOI: 10.17586/1606-4313-2024-23-4-46-51
Abstract
The search for methods demonstrating the preservation of the structure of β-glucans after their isolation is an urgent practical problem. The aim of the study was to evaluate the characteristics of β-glucans from the fruit bodies of Pleurotus ostreatus and the mycelium Phallus impudicus using IR spectroscopy. During the research, samples containing soluble and insoluble fractions of β-glucans were obtained. The content of β-glucans in the samples was determined using an enzymatic method. The presence of α- and β-glycosidic bonds was determined using IR spectroscopy. It has been shown that the IR spectroscopy method is a promising tool for the structural analysis of polysaccharides, due to the possibility of recording the glycosidic bonds of α- and β-glucans. This method can be recommended for the identification and standardization of fungal polysaccharides obtained from the mycelium and fruiting bodies of basidiomycetes.
Abstract
The search for methods demonstrating the preservation of the structure of β-glucans after their isolation is an urgent practical problem. The aim of the study was to evaluate the characteristics of β-glucans from the fruit bodies of Pleurotus ostreatus and the mycelium Phallus impudicus using IR spectroscopy. During the research, samples containing soluble and insoluble fractions of β-glucans were obtained. The content of β-glucans in the samples was determined using an enzymatic method. The presence of α- and β-glycosidic bonds was determined using IR spectroscopy. It has been shown that the IR spectroscopy method is a promising tool for the structural analysis of polysaccharides, due to the possibility of recording the glycosidic bonds of α- and β-glucans. This method can be recommended for the identification and standardization of fungal polysaccharides obtained from the mycelium and fruiting bodies of basidiomycetes.
Keywords: basidiomycetes, β-glucan, Pleurotus ostreatus, Phallus impudicus, enzymatic method, IR spectroscopy.
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